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GlycoFlux™ MSCV-Human LDHB Viral Particle (CAT#: GLJF-1025-JF699)

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Description GlycoFlux™ MSCV-LDHB Viral Particle ensures lasting LDHB expression with MSCV, favoring oxidative lactate utilization (lactate→pyruvate) and enabling analysis of the peroxisome-targeted readthrough isoform. Track LDH isozyme balance (H vs M subunits), oxygen consumption, and lactate clearance.
Product Type Recombinant MSCV Retrovirus
Target LDHB
Species Human
Promotor MSCV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.
Shipping Dispatched on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to preserve titer and stability.
Handling Notes Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.
Full Name Lactate Dehydrogenase B
Alternative Name LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
Location 12p12.1
Gene ID 3945
Summary This gene encodes the B chain of lactate dehydrogenase, responsible for the reversible interconversion of pyruvate and lactate, alongside NADH and NAD+, following glycolysis. Multiple transcript variants exist, including a C-terminally extended isoform generated via stop codon readthrough, which localizes to peroxisomes. Mutations cause lactate dehydrogenase B deficiency. Pseudogenes are present on chromosomes X, 5, and 13.
For Research Use Only.
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