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The GlycoSens™ Endogenous Carbon Monoxide (CO) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of endogenous carbon monoxide (CO) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Quantification of endogenous carbon monoxide (CO) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Found widely across biological samples, endogenous carbon monoxide (CO) is crucial for maintaining systemic homeostasis. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.
Application
Ideal for investigating endogenous carbon monoxide (CO) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
Key Features & Advantages
High Specificity; Excellent Reproducibility; Stringent Quality Control
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.