GlycoSens™ Phosphatidate Phosphatase (PPase) Assay Kit

The GlycoSens™ Phosphatidate Phosphatase (PPase) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of phosphatidate phosphatase (PPase) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Fatty Acid Series

PPase

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

Quantification of phosphatidate phosphatase (PPase) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Phosphatidate Phosphatase

Phosphatidate Phosphatase; PPase

Phosphatidate phosphatase (PPase) is an integral element required for robust cellular function and metabolism. It plays a crucial part in the mobilization of organic phosphorus and metabolic regulation. Thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Ideal for investigating phosphatidate phosphatase (PPase) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Linear Dynamic Range; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.