GlycoSens™ Phosphatidate Phosphatase (PPase) Assay Kit, Microplate Method

The GlycoSens™ Phosphatidate Phosphatase (PPase) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of phosphatidate phosphatase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Other Series

PPase

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

20 μL - 100 μL

Target phosphatidate phosphatase (PPase) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target PPase.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Phosphatidate Phosphatase

Phosphatidate Phosphatase; PPase

Functioning at the core of various biological processes, phosphatidate phosphatase (PPase) is a key subject of biochemical analysis. It plays a crucial part in the mobilization of organic phosphorus and metabolic regulation. Monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Ideal for investigating phosphatidate phosphatase (PPase) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.