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GlycoSens™ Phosphatidate Phosphatase (PPase) Assay Kit, Spectrophotometry

CAT#: GLJF-0926-HX1415

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The GlycoSens™ Phosphatidate Phosphatase (PPase) Assay Kit offers an advanced quantitative methodology for evaluating phosphatidate phosphatase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Other Series
Target
PPase
Kit Components
Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
The system measures chromatic product formation derived from target phosphatidate phosphatase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of PPase levels.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Phosphatidate Phosphatase
Alternate Names
Phosphatidate Phosphatase; PPase
Application Notes
Background
Phosphatidate phosphatase (PPase) acts as an essential biochemical entity within diverse cellular pathways. This enzyme enables the dephosphorylation of target molecules, fundamentally altering their functional state. As a result, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.
Application
Tailored for accurate quantification of phosphatidate phosphatase (PPase) across cell biology, organelle bioenergetics, and intracellular signaling cascades.
Key Features & Advantages
Rapid Reaction Protocol; High Signal-to-Noise Ratio; Enhanced Sensitivity
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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