Immobilized Trypsin (Agarose Resin) Treated with TPCK

Immobilized trypsin (agarose resin) treated with TPCK is a product that immobilizes bovine trypsin on an agarose matrix and is provided as a 50% slurry. Trypsin is a serine protease that specifically hydrolyzes proteins, particularly at the carboxyl side of cysteine, arginine, and lysine residues to generate peptides that can be analyzed by mass spectrometry. To avoid interference with sequence analysis by chymotrypsin, our immobilized trypsin has been treated with TPCK to effectively inactivate chymotrypsin. Using this immobilized form of trypsin, peptide bond cleavage can be performed without enzyme contamination.

Immobilized trypsin, TPCK-treated (agarose resin); EC 3.4.21.4; TPCK-treated trypsin agarose; Agarose-bound trypsin, TPCK-treated; Trypsin (TPCK-treated) immobilized on agarose; Agarose-trypsin conjugate (TPCK-treated)

Bovine

The activity level is at least 200 TAME units per milliliter of resin. One unit corresponds to the hydrolysis of 1 micromole of TAME (p-toluenesulfonyl-L-arginine methyl ester) per minute under the conditions of pH 8.2 and a temperature of 25°C.

Mass spectrometry

Slurry

Store at 2-8°C for long term storage.

Immobilized trypsin (agarose resin) treated with TPCK is mainly used for highly specific enzymatic hydrolysis of proteins, and is particularly suitable for the preparation of peptides for mass spectrometry analysis in proteomics research. Since trypsin is immobilized on agarose, it is easy to separate from the product after enzymatic hydrolysis, avoiding the interference of free enzyme on subsequent analysis and improving the accuracy of the experiment. TPCK treatment also inhibits the possible activity of chymotrypsin, ensuring the specificity of enzymatic hydrolysis.