L-Lysine-2HCl, ¹³C₆ for SILAC

L-Lysine-2HCl, ¹³C₆ for SILAC is a specially designed, isotopically labeled form of the essential amino acid L-lysine. In this compound, all six carbon atoms within the lysine molecule are replaced with the stable isotope carbon-13, resulting in a molecule where the lysine is uniformly labeled. The addition of two hydrochloride groups forms the dihydrochloride salt, enhancing the compound's solubility and stability in aqueous solutions. This product is specifically designed for use in stable isotope labeling by amino acids in cell culture (SILAC), a powerful technique used in quantitative proteomics. Incorporating this ¹³C₆-labeled lysine into cell culture media allows for the metabolic incorporation of the heavier isotope into all proteins synthesized by the cells. This creates a distinct mass difference between proteins from labeled and unlabeled cell populations, enabling researchers to distinguish and quantify relative protein abundance between different experimental conditions using mass spectrometry.

201740-81-0

201740-81-0; L-Lysine-2HCl, ¹³C₆; L-Lysine-¹³C₆ dihydrochloride; (2S)-2,6-Diamino(1,2,3,4,5,6-¹³C₆)hexanoic acid;dihydrochloride

C₆H₁₆C₁₂N₂O₂

225.06

InChI=1S/C6H14N2O2.2ClH/c7-4-2-1-3-5(8)6(9)10;;/h5H,1-4,7-8H2,(H,9,10);2*1H/t5-;;/m0../s1/i1+1,2+1,3+1,4+1,5+1,6+1;

JBBURJFZIMRPCZ-NHMLGGAVSA-N

Min. 95%

Conforms to structure

Store at 2-8°C for long term storage.

L-Lysine-2HCl, ¹³C₆ for SILAC is widely used to study changes in protein expression, post-translational modifications, and protein-protein interactions. It is a crucial tool for advancing our understanding of cellular processes, disease mechanisms, and the development of novel therapeutic strategies.