Chymotrypsin Protease, Sequencing Grade (CAT#: GLJF-0225-HX21)

Product Description	Chymotrypsin protease (sequencing grade) is a highly purified serine endonuclease derived from bovine pancreas. It specifically cleaves the carboxyl termini of tyrosine, tryptophan, and phenylalanine. The enzyme exhibits optimal activity in the pH range of 7.0-9.0 and can be used alone or in combination with other proteases for digestion processes in solution or in gel.
Synonyms	Chymotrypsin, sequencing grade; Sequencing grade chymotrypsin; Chymotrypsin for protein sequencing; High-purity chymotrypsin for sequencing
MW	25 kDa
Source	Bovine pancreas
Purity	Min. 90%
Activity	When assessed using the BTEE assay (N-benzoyl-L-tyrosine ethyl ester), the enzyme has a specific activity of no less than 70 units per milligram. One unit is defined as the amount of chymotrypsin that hydrolyzes 1.0 micromole of BTEE per minute under conditions of pH 7.8 and 25°C.
Form	Lyophilized powder
Storage	Store at -20°C for long term storage.

Application

Chymotrypsin protease (sequencing grade) is a highly purified proteolytic enzyme with excellent specificity and stable enzyme activity, which is widely used in the fields of proteomics and bioanalysis. In mass spectrometry analysis, it is used to specifically cut complex protein samples into smaller peptides, especially hydrolyzing the carboxyl termini of aromatic amino acids such as tyrosine, tryptophan and phenylalanine. By combining with trypsin, sequencing-grade chymotrypsin can improve the coverage and accuracy of protein identification, helping researchers to deeply analyze the structure and functions of proteins. The high purity and reliability of this enzyme ensure the reproducibility of experimental results, and it is a key tool for protein analysis and identification.