L-Leucine, ¹³C₆ for SILAC

L-Leucine, ¹³C₆ for SILAC is a stable isotope-labeled form of the essential amino acid L-leucine, wherein all six carbon atoms are replaced with the stable isotope carbon-13. This fully labeled amino acid is crucial for stable isotope labeling by amino acids in cell culture (SILAC) experiments, a quantitative proteomics technique. By supplementing cell culture media with L-leucine, ¹³C₆, researchers enable cells to incorporate the labeled leucine into newly synthesized proteins. This incorporation results in a predictable mass shift detectable by mass spectrometry, allowing for precise differentiation and quantification of proteins from different experimental conditions.

201740-84-3

201740-84-3; (2S)-2-Amino-4-(113C)methyl(1,2,3,4,5-13C5)pentanoic acid; L-Leucine-¹³C₆

C₆H₁₃NO₂

137.13

InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1/i1+1,2+1,3+1,4+1,5+1,6+1

ROHFNLRQFUQHCH-IQUPZBQQSA-N

Min. 95%

Conforms to structure

Store at 2-8°C for long term storage.

L-Leucine, ¹³C₆ for SILAC is a stable isotope-labeled form of the amino acid L-leucine. It is used in SILAC experiments, a mass spectrometry-based technique for quantitative proteomics. In SILAC, cells are grown in a medium containing L-leucine, ¹³C₆, which is incorporated into all newly synthesized proteins. This results in a "heavy" form of the protein, which can be distinguished from the "light" form of the protein (from cells grown in a normal medium) by mass spectrometry. SILAC is used to compare protein expression levels between different cell states, such as normal and diseased cells.