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L-Leucine, 13C6 for SILAC (CAT#: GLJF-0225-HX31)

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250 mg

Product Description L-Leucine, 13C6 for SILAC is a stable isotope-labeled form of the essential amino acid L-leucine, wherein all six carbon atoms are replaced with the stable isotope carbon-13. This fully labeled amino acid is crucial for stable isotope labeling by amino acids in cell culture (SILAC) experiments, a quantitative proteomics technique. By supplementing cell culture media with L-leucine, 13C6, researchers enable cells to incorporate the labeled leucine into newly synthesized proteins. This incorporation results in a predictable mass shift detectable by mass spectrometry, allowing for precise differentiation and quantification of proteins from different experimental conditions.
CAS 201740-84-3
Synonyms 201740-84-3; (2S)-2-Amino-4-(113C)methyl(1,2,3,4,5-13C5)pentanoic acid; L-Leucine-13C6
Formula C6H13NO2
MW 137.13
InChI InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1/i1+1,2+1,3+1,4+1,5+1,6+1
InChI Key ROHFNLRQFUQHCH-IQUPZBQQSA-N
Purity Min. 95%
Identity Conforms to structure
Storage Store at 2-8°C for long term storage.
Application L-Leucine, 13C6 for SILAC is a stable isotope-labeled form of the amino acid L-leucine. It is used in SILAC experiments, a mass spectrometry-based technique for quantitative proteomics. In SILAC, cells are grown in a medium containing L-leucine, 13C6, which is incorporated into all newly synthesized proteins. This results in a "heavy" form of the protein, which can be distinguished from the "light" form of the protein (from cells grown in a normal medium) by mass spectrometry. SILAC is used to compare protein expression levels between different cell states, such as normal and diseased cells.
For Research Use Only.
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