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Quantitative Human ADCY5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX120

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Specifications
Product Description
The quantitative human ADCY5 (glycosylated) sandwich ELISA kit is designed to detect human adenylate cyclase 5 (ADCY5) levels. ADCY5 is an enzyme responsible for catalyzing the conversion of ATP to cyclic AMP (cAMP), a crucial second messenger involved in various cellular processes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.151 ng/mL, which can accurately detect low concentrations of ADCY5 in the sample.
Target
ADCY5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.151 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-20 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Adenylate cyclase 5
Alternate Names
ADCY5; Adenylate cyclase 5; Adenylate cyclase type V
Uniprot No.
N-Glycosylation Site
870, 887
Application Notes
Application
The quantitative human ADCY5 (glycosylated) sandwich ELISA kit is valuable for research investigating ADCY5's role in signal transduction, metabolic regulation, and diseases such as diabetes and movement disorders, allowing researchers to quantify ADCY5 levels and better understand its function in those disease processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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