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Quantitative Human APOB100 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX165)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human APOB100 (glycosylated) sandwich ELISA kit is designed to detect human apolipoprotein B100 (APOB100) levels. APOB100 is a large protein isoform of apolipoprotein B, essential for the assembly and secretion of very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL), playing a critical role in cholesterol transport. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 9.026 ng/mL, which can accurately detect low concentrations of APOB100 in the sample.
Target APOB100
N-Glycosylation Site 34, 185, 983, 1368, 1377, 1523, 2239, 2560, 2779, 2982, 3101, 3224, 3336, 3358, 3411, 3465, 3895, 4237, 4431
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 9.026 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 25 ng/mL-1250 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Apolipoprotein B100
Alternate Names APOB100; Apolipoprotein B100; Apo-B100
Uniprot No. P04114
Application The quantitative human APOB100 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for assessing cardiovascular risk, monitoring lipid metabolism disorders, and evaluating the efficacy of lipid-lowering therapies. Researchers and clinicians utilize this ELISA to quantify APOB100 levels, providing insights into LDL cholesterol metabolism and potential therapeutic interventions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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