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Quantitative Human APOB100 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX165

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Specifications
Product Description
The quantitative human APOB100 (glycosylated) sandwich ELISA kit is designed to detect human apolipoprotein B100 (APOB100) levels. APOB100 is a large protein isoform of apolipoprotein B, essential for the assembly and secretion of very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL), playing a critical role in cholesterol transport. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 9.026 ng/mL, which can accurately detect low concentrations of APOB100 in the sample.
Target
APOB100
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
9.026 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
25 ng/mL-1250 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Apolipoprotein B100
Alternate Names
APOB100; Apolipoprotein B100; Apo-B100
Uniprot No.
N-Glycosylation Site
34, 185, 983, 1368, 1377, 1523, 2239, 2560, 2779, 2982, 3101, 3224, 3336, 3358, 3411, 3465, 3895, 4237, 4431
Application Notes
Application
The quantitative human APOB100 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for assessing cardiovascular risk, monitoring lipid metabolism disorders, and evaluating the efficacy of lipid-lowering therapies. Researchers and clinicians utilize this ELISA to quantify APOB100 levels, providing insights into LDL cholesterol metabolism and potential therapeutic interventions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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