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Quantitative Human ASPH (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX547

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Specifications
Product Description
The quantitative human ASPH (glycosylated) sandwich ELISA kit is designed to detect human aspartate β hydroxylase (ASPH) levels. ASPH is an enzyme that catalyzes the post-translational hydroxylation of aspartate and asparagine residues in certain proteins. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.077 ng/mL, which can accurately detect low concentrations of ASPH in the sample.
Target
ASPH
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.077 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Aspartate β hydroxylase
Alternate Names
ASPH; Aspartate β hydroxylase; BAH; JCTN; Junctin; Peptide-aspartate β-dioxygenase
Uniprot No.
N-Glycosylation Site
64, 452, 706
Application Notes
Application
The quantitative human ASPH (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and biochemistry, particularly in studies of post-translational modifications. Researchers use it to measure ASPH levels in tissues. The kit has applications in studies of cancer and developmental biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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