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Quantitative Human CHRNα7 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX392

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Specifications
Product Description
The quantitative human CHRNα7 (glycosylated) sandwich ELISA kit is designed to detect human cholinergic receptor, nicotinic, α7 (CHRNα7) levels. CHRNα7 is a subunit of the nicotinic acetylcholine receptor. This receptor is a ligand-gated ion channel. CHRNα7 plays a role in neurotransmission. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.127 ng/mL, which can accurately detect low concentrations of CHRNα7 in the sample.
Target
CHRNα7
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.127 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Cholinergic receptor, nicotinic, α7
Alternate Names
CHRNα7; Cholinergic receptor, nicotinic, α7; CHRN-A7; NAChRA7; Neuronal acetylcholine receptor α7
Uniprot No.
N-Glycosylation Site
46, 90, 133
Application Notes
Application
The quantitative human CHRNα7 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CHRNα7 levels. It is useful for studying the role of alpha7 nicotinic receptors in synaptic transmission.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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