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Quantitative Human COL11α1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX232

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Specifications
Product Description
The quantitative human COL11α1 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 11 α1 (COL11α1) levels. COL11α1 is a fibrillar collagen subunit that contributes to the formation of heterotypic collagen fibrils, particularly in cartilage and other connective tissues. It plays a crucial role in regulating fibril diameter and organization. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 16.902 pg/mL, which can accurately detect low concentrations of COL11α1 in the sample.
Target
COL11α1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
16.902 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 pg/mL-2000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Collagen type 11 α1
Alternate Names
COL11α1; Collagen type 11 α1; COL11-A1; COLL6; Collagen α1(XI) chain
Uniprot No.
N-Glycosylation Site
1640
Application Notes
Application
The quantitative human COL11α1 (glycosylated) sandwich ELISA kit is valuable in research investigating cartilage development, osteoarthritis, and other connective tissue disorders. Researchers utilize this ELISA to quantify COL11α1 levels, providing insights into its role in extracellular matrix remodeling and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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