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Quantitative Human CPA4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX881

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Specifications
Product Description
The quantitative human CPA4 (glycosylated) sandwich ELISA kit is designed to detect human carboxypeptidase A4 (CPA4) levels. CPA4 is a metalloprotease that removes C-terminal amino acids from proteins. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.093 ng/mL, which can accurately detect low concentrations of CPA4 in the sample.
Target
CPA4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.093 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Carboxypeptidase A4
Alternate Names
CPA4; Carboxypeptidase A4; CBPA4_HUMAN; Cpa4
Uniprot No.
N-Glycosylation Site
260
Application Notes
Application
The quantitative human CPA4 (glycosylated) sandwich ELISA kit is used to measure CPA4 levels in biological samples. This kit is applicable in studies examining protein processing and hormone regulation. It is also relevant to studies investigating the role of CPA4 in modulating peptide hormone levels and its potential involvement in various physiological processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-105%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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