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Quantitative Human DCT (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX399

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Specifications
Product Description
The quantitative human DCT (glycosylated) sandwich ELISA kit is designed to detect human dopachrome tautomerase (DCT) levels. DCT is an enzyme, which is involved in melanin synthesis. DCT plays a role in determining skin pigmentation. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.138 ng/mL, which can accurately detect low concentrations of DCT in the sample.
Target
DCT
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.138 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Dopachrome tautomerase
Alternate Names
DCT; Dopachrome tautomerase; TYRP2; TRP-2; Tyrosine-related protein 2
Uniprot No.
N-Glycosylation Site
170, 178, 237, 300, 342, 377
Application Notes
Application
The quantitative human DCT (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated DCT levels. It is useful for studying the role of DCT in melanogenesis.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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