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Quantitative Human DCT (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX399)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human DCT (glycosylated) sandwich ELISA kit is designed to detect human dopachrome tautomerase (DCT) levels. DCT is an enzyme, which is involved in melanin synthesis. DCT plays a role in determining skin pigmentation. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.138 ng/mL, which can accurately detect low concentrations of DCT in the sample.
Target DCT
N-Glycosylation Site 170, 178, 237, 300, 342, 377
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.138 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Dopachrome tautomerase
Alternate Names DCT; Dopachrome tautomerase; TYRP2; TRP-2; Tyrosine-related protein 2
Uniprot No. P40126
Application The quantitative human DCT (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated DCT levels. It is useful for studying the role of DCT in melanogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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