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Quantitative Human EAAT5 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX12)

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1×48 T
1×96 T

Product Description The quantitative human EAAT5 (glycosylated) sandwich ELISA kit is designed to detect human excitatory amino acid transporter 5 (EAAT5) levels. EAAT5 is a glutamate transporter primarily expressed in the retina, where it plays a critical role in regulating glutamate levels at synapses. It contributes to synaptic transmission and protects retinal neurons from excitotoxicity. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.132 ng/mL, which can accurately detect low concentrations of EAAT5 in the sample.
Target EAAT5
N-Glycosylation Site 191
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.132 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Excitatory amino acid transporter 5
Alternate Names EAAT5; Excitatory amino acid transporter 5; SLC1A7; Solute carrier family 1 member 7, glutamate transporter
Uniprot No. O00341
Application The quantitative human EAAT5 (glycosylated) sandwich ELISA kit is valuable for researchers investigating retinal diseases, such as glaucoma and diabetic retinopathy, where glutamate homeostasis is disrupted. By quantifying EAAT5 levels, researchers can gain insights into the role of this transporter in disease pathogenesis and explore potential therapeutic interventions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 90-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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