Quantitative Human EAAT5 (Glycosylated) Sandwich ELISA Kit

The quantitative human EAAT5 (glycosylated) sandwich ELISA kit is designed to detect human excitatory amino acid transporter 5 (EAAT5) levels. EAAT5 is a glutamate transporter primarily expressed in the retina, where it plays a critical role in regulating glutamate levels at synapses. It contributes to synaptic transmission and protects retinal neurons from excitotoxicity. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.132 ng/mL, which can accurately detect low concentrations of EAAT5 in the sample.

EAAT5

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.132 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Excitatory amino acid transporter 5

EAAT5; Excitatory amino acid transporter 5; SLC1A7; Solute carrier family 1 member 7, glutamate transporter

191

The quantitative human EAAT5 (glycosylated) sandwich ELISA kit is valuable for researchers investigating retinal diseases, such as glaucoma and diabetic retinopathy, where glutamate homeostasis is disrupted. By quantifying EAAT5 levels, researchers can gain insights into the role of this transporter in disease pathogenesis and explore potential therapeutic interventions.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-110%;
Plasma sample: n=4, 90-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.