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Quantitative Human ERFE (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX625)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ERFE (glycosylated) sandwich ELISA kit is designed to detect human erythroferrone (ERFE) levels. ERFE is a hormone produced by erythroblasts, which are red blood cell precursors, in response to erythropoietin (EPO). The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.069 ng/mL, which can accurately detect low concentrations of ERFE in the sample.
Target ERFE
N-Glycosylation Site 243, 295, 333
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.069 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Erythroferrone
Alternate Names ERFE; Erythroferrone; C1QTNF15; CTRP15; Complement C1q tumor necrosis factor-related protein 15
Uniprot No. Q4G0M1
Application The quantitative human ERFE (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ERFE concentration in biological samples. The kit finds application in studies related to erythropoiesis, iron metabolism, and anemia. It is valuable in fields such as hematology and nephrology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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