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Quantitative Human ERFE (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX625

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Specifications
Product Description
The quantitative human ERFE (glycosylated) sandwich ELISA kit is designed to detect human erythroferrone (ERFE) levels. ERFE is a hormone produced by erythroblasts, which are red blood cell precursors, in response to erythropoietin (EPO). The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.069 ng/mL, which can accurately detect low concentrations of ERFE in the sample.
Target
ERFE
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.069 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Erythroferrone
Alternate Names
ERFE; Erythroferrone; C1QTNF15; CTRP15; Complement C1q tumor necrosis factor-related protein 15
Uniprot No.
N-Glycosylation Site
243, 295, 333
Application Notes
Application
The quantitative human ERFE (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ERFE concentration in biological samples. The kit finds application in studies related to erythropoiesis, iron metabolism, and anemia. It is valuable in fields such as hematology and nephrology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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