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Quantitative Human FGF3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX231

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Specifications
Product Description
The quantitative human FGF3 (glycosylated) sandwich ELISA kit is designed to detect human fibroblast growth factor 3 (FGF3) levels. FGF3 is a member of the fibroblast growth factor family, involved in embryonic development, particularly inner ear and limb development, and plays a role in cell proliferation and differentiation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 5.824 pg/mL, which can accurately detect low concentrations of FGF3 in the sample.
Target
FGF3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
5.824 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
25 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Fibroblast growth factor 3
Alternate Names
FGF3; Fibroblast growth factor 3; HBGF3; INT2; Heparin-binding growth factor 3
Uniprot No.
N-Glycosylation Site
65
Application Notes
Application
The quantitative human FGF3 (glycosylated) sandwich ELISA kit is valuable in research investigating developmental biology, cancer biology, and hearing disorders. Researchers utilize this ELISA to quantify FGF3 levels, providing insights into its role in these processes and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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