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Quantitative Human GRIN2A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX552

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Specifications
Product Description
The quantitative human GRIN2A (glycosylated) sandwich ELISA kit is designed to detect human glutamate receptor, ionotropic, N-methyl-D-aspartate 2A (GRIN2A) levels. GRIN2A is a subunit of the NMDA receptor. NMDA receptors are ligand-gated ion channels found in nerve cells. They are critical for synaptic transmission, learning, and memory. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of GRIN2A in the sample.
Target
GRIN2A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glutamate receptor, ionotropic, N-methyl-D-aspartate 2A
Alternate Names
GRIN2A; Glutamate receptor, ionotropic, N-methyl-D-aspartate 2A; NMDAR2A; NR2A; N-Methyl D-aspartate receptor subtype 2A
Uniprot No.
N-Glycosylation Site
75, 340, 380, 443, 444, 541, 687
Application Notes
Application
The quantitative human GRIN2A (glycosylated) sandwich ELISA kit is a valuable tool for research in neuroscience and neuropharmacology, particularly in studies of glutamate signaling and synaptic plasticity. Researchers use it to measure GRIN2A levels in brain tissue. The kit has applications in studies of neurological disorders, learning, and memory.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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