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Quantitative Human HS2ST1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX662)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human HS2ST1 (glycosylated) sandwich ELISA kit is designed to detect human heparan sulfate-2-O-sulfotransferase 1 (HS2ST1) levels. HS2ST1 is an enzyme involved in the biosynthesis of heparan sulfate (HS). HS2ST1 catalyzes the transfer of sulfate groups to specific positions within the heparan sulfate chain. This sulfation is crucial for the ability of HS to interact with various proteins and regulate diverse biological processes. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.084 ng/mL, which can accurately detect low concentrations of HS2ST1 in the sample.
Target HS2ST1
N-Glycosylation Site 108, 127
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.084 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Heparan sulfate-2-O-sulfotransferase 1
Alternate Names HS2ST1; Heparan sulfate-2-O-sulfotransferase 1; 2-O-Sulfotransferase; HS2ST_HUMAN
Uniprot No. Q7LGA3
Application The quantitative human HS2ST1 (glycosylated) sandwich ELISA kit is used to quantitatively measure glycosylated HS2ST1 concentrations in biological samples. It proves valuable in research investigating heparan sulfate biosynthesis, cell signaling, and the role of HS in various physiological and pathological processes. This kit finds application in fields such as biochemistry, cell biology, and glycobiology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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