Quantitative Human HS2ST1 (Glycosylated) Sandwich ELISA Kit

The quantitative human HS2ST1 (glycosylated) sandwich ELISA kit is designed to detect human heparan sulfate-2-O-sulfotransferase 1 (HS2ST1) levels. HS2ST1 is an enzyme involved in the biosynthesis of heparan sulfate (HS). HS2ST1 catalyzes the transfer of sulfate groups to specific positions within the heparan sulfate chain. This sulfation is crucial for the ability of HS to interact with various proteins and regulate diverse biological processes. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.084 ng/mL, which can accurately detect low concentrations of HS2ST1 in the sample.

HS2ST1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.084 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Heparan sulfate-2-O-sulfotransferase 1

HS2ST1; Heparan sulfate-2-O-sulfotransferase 1; 2-O-Sulfotransferase; HS2ST_HUMAN

108, 127

The quantitative human HS2ST1 (glycosylated) sandwich ELISA kit is used to quantitatively measure glycosylated HS2ST1 concentrations in biological samples. It proves valuable in research investigating heparan sulfate biosynthesis, cell signaling, and the role of HS in various physiological and pathological processes. This kit finds application in fields such as biochemistry, cell biology, and glycobiology.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.