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Quantitative Human IL5Rα (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX510

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Specifications
Product Description
The quantitative human IL5Rα (glycosylated) sandwich ELISA kit is designed to detect human interleukin 5 receptor α (IL5Rα) levels. IL5Rα is a protein that forms part of the receptor complex for interleukin-5 (IL-5). IL-5 is a cytokine that plays a crucial role in the differentiation, activation, and survival of eosinophils. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 25.826 pg/mL, which can accurately detect low concentrations of IL5Rα in the sample.
Target
IL5Rα
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
25.826 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
100 pg/mL-4000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Interleukin 5 receptor α
Alternate Names
IL5Rα; Interleukin 5 receptor α; CD125; IL5-RA; HSIL5R3; IL5R
Uniprot No.
N-Glycosylation Site
35, 131, 216, 244
Application Notes
Application
The quantitative human IL5Rα (glycosylated) sandwich ELISA kit is a valuable tool for immunology research, particularly in studies of eosinophil biology and allergic diseases. Researchers use it to measure IL5Rα levels in biological samples. The kit has applications in studies of asthma, allergies, and parasitic infections, as well as in the development of therapies targeting IL-5 signaling.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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