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Quantitative Human ISM1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX7

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Specifications
Product Description
The quantitative human ISM1 (glycosylated) sandwich ELISA kit is designed to detect human isthmin 1 (ISM1 ) levels. ISM1 is a secreted protein involved in angiogenesis, cell adhesion, and immune regulation. It plays a role in various physiological processes, including embryonic development and tumor suppression. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, plasma. Its sensitivity is 0.085 ng/mL, which can accurately detect low concentrations of ISM1 in the sample.
Target
ISM1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, plasma
Sample Volume
100 μL
Sensitivity
0.085 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.50 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Isthmin 1
Alternate Names
ISM1; Isthmin 1; ISM; Isthmin; C20orf82
Uniprot No.
N-Glycosylation Site
39
Application Notes
Application
The quantitative human ISM1 (glycosylated) sandwich ELISA kit is useful for researchers investigating ISM1's role in diseases such as cancer, vascular disorders, and inflammatory conditions, as it allows for the quantification of ISM1 in biological samples. The data obtained from this kit can help determine the correlation between ISM1 levels and disease progression or treatment response.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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