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Quantitative Human JAM1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX948

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Specifications
Product Description
The quantitative human JAM1 (glycosylated) sandwich ELISA kit is designed to detect human junctional adhesion molecule 1 (JAM1) levels. JAM1 is a transmembrane protein that is expressed at intercellular junctions. These junctions are located between endothelial cells and epithelial cells. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.155 ng/mL, which can accurately detect low concentrations of JAM1 in the sample.
Target
JAM1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.155 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.55 ng/mL-12.50 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Junctional adhesion molecule 1
Alternate Names
JAM1; Junctional adhesion molecule 1; CD321; F11R; JAMA; PAM1; Coagulation factor XI receptor; Platelet F11 receptor
Uniprot No.
N-Glycosylation Site
185
Application Notes
Application
The quantitative human JAM1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated JAM1 levels in biological samples. The assay is valuable for investigating the role of JAM1 in cell adhesion, vascular biology, and inflammatory responses.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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