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Quantitative Human LAMβ1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX193)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human LAMβ1 (glycosylated) sandwich ELISA kit is designed to detect human laminin β1 (LAMβ1) levels. LAMβ1 is a subunit of laminin, a major component of the basement membrane, playing a crucial role in cell adhesion, migration, and differentiation. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 14.027 pg/mL, which can accurately detect low concentrations of LAMβ1 in the sample.
Target LAMβ1
N-Glycosylation Site 120, 356, 519, 677, 1041, 1195, 1279, 1336, 1343, 1487, 1542
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 14.027 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 35 pg/mL-1500 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Laminin β1
Alternate Names LAMβ1; Laminin β1; LAM-B1
Uniprot No. P07942
Application The quantitative human LAMβ1 (glycosylated) sandwich ELISA kit is valuable in research investigating extracellular matrix remodeling, wound healing, and diseases involving basement membrane dysfunction, such as cancer and kidney disease. Researchers utilize this ELISA to quantify LAMβ1 levels, providing insights into its role in tissue integrity and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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