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Quantitative Human LAMC2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX576

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Specifications
Product Description
The quantitative human LAMC2 (glycosylated) sandwich ELISA kit is designed to detect human laminin γ2 (LAMC2) levels. LAMC2 is another protein that is a subunit of laminin. Laminins are key components of basement membranes, which are thin layers of extracellular matrix underlying epithelial and endothelial cells. LAMC2 plays a crucial role in the assembly and functions of these membranes, influencing cell adhesion, migration, and differentiation. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 7.102 pg/mL, which can accurately detect low concentrations of LAMC2 in the sample.
Target
LAMC2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
7.102 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 pg/mL-800 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Laminin γ2
Alternate Names
LAMC2; Laminin γ2; EBR2A; LAMB2T
Uniprot No.
N-Glycosylation Site
342, 362, 942, 1033
O-Glycosylation Site
803, 805
Application Notes
Application
The quantitative human LAMC2 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and extracellular matrix biology, particularly in the study of basement membranes. Researchers can utilize it to measure LAMC2 levels in various tissue samples. The kit has applications in studies of wound healing, skin diseases, and cancer metastasis, where basement membrane dynamics are critical.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-90%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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