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Quantitative Human LPAR1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX716

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Specifications
Product Description
The quantitative human LPAR1 (glycosylated) sandwich ELISA kit is designed to detect human lysophosphatidic acid receptor 1 (LPAR1) levels. LPAR1 is a G protein-coupled receptor, which is activated by lysophosphatidic acid (LPA). The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of LPAR1 in the sample.
Target
LPAR1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Lysophosphatidic acid receptor 1
Alternate Names
LPAR1; Lysophosphatidic acid receptor 1; EDG2; LPA1
Uniprot No.
N-Glycosylation Site
27, 35
Application Notes
Application
The quantitative human LPAR1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LPAR1 in samples. This quantification is important for research on LPA signaling and cell growth. The kit also has potential applications in investigations of cancer.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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