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Quantitative Human MAdCAM1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX568

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Specifications
Product Description
The quantitative human MAdCAM1 (glycosylated) sandwich ELISA kit is designed to detect human mucosal addressin cell adhesion molecule 1 (MAdCAM1) levels. MAdCAM1 is a cell adhesion molecule, which is expressed on endothelial cells in the gut-associated lymphoid tissue. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma, urine . Its sensitivity is 38.034 pg/mL, which can accurately detect low concentrations of MAdCAM1 in the sample.
Target
MAdCAM1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma, urine
Sample Volume
100 μL
Sensitivity
38.034 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
100 pg/mL-4000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Mucosal addressin cell adhesion molecule 1
Alternate Names
MAdCAM1; Mucosal addressin cell adhesion molecule 1
Uniprot No.
N-Glycosylation Site
83
Application Notes
Application
The quantitative human MAdCAM1 (glycosylated) sandwich ELISA kit is a valuable tool for research in immunology and gastroenterology, particularly in studies of lymphocyte trafficking. Researchers can measure MAdCAM1 levels in tissues and cell culture. Applications include studies of inflammatory bowel disease and gut immunity.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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