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Quantitative Human NID2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX584)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NID2 (glycosylated) sandwich ELISA kit is designed to detect human nidogen 2 (NID2) levels. NID2 is a protein found in basement membranes, which is a key component of these specialized extracellular matrices. It plays a role in linking different basement membrane components. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.093 ng/mL, which can accurately detect low concentrations of NID2 in the sample.
Target NID2
N-Glycosylation Site 417, 658, 693, 703, 1124
O-Glycosylation Site 358, 359, 452
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.093 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Nidogen 2
Alternate Names NID2; Nidogen 2; Osteonidogen
Uniprot No. Q14112
Application The quantitative human NID2 (glycosylated) sandwich ELISA kit is designed to quantitatively measure NID2 levels in biological specimens. This measurement is essential for studies investigating basement membrane structure. It also contributes to understanding the role of Nidogen-2 in tissue organization.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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