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Quantitative Human P4Hα2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX49)

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1×48 T
1×96 T

Product Description The quantitative human P4Hα2 (glycosylated) sandwich ELISA kit is designed to detect human prolyl-4-hydroxylase α polypeptide 2 (P4Hα2) levels. P4Hα2 is a subunit of prolyl-4-hydroxylase, an enzyme crucial for collagen synthesis by catalyzing the formation of 4-hydroxyproline residues in collagen molecules. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of P4Hα2 in the sample.
Target P4Hα2
N-Glycosylation Site 115, 264
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.120 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Prolyl-4-hydroxylase α polypeptide 2
Alternate Names P4Hα2; Prolyl-4-hydroxylase α polypeptide 2; Alpha polypeptide II
Uniprot No. O15460
Application The quantitative human P4Hα2 (glycosylated) sandwich ELISA kit utilizes a sandwich ELISA format, allowing researchers to measure P4Hα2 levels, which is valuable in studies related to collagen biosynthesis, fibrosis, and cancer progression. This assay serves as a tool for investigating the role of P4Hα2 in various physiological and pathological processes.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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