Quantitative Human P4Hα2 (Glycosylated) Sandwich ELISA Kit

The quantitative human P4Hα2 (glycosylated) sandwich ELISA kit is designed to detect human prolyl-4-hydroxylase α polypeptide 2 (P4Hα2) levels. P4Hα2 is a subunit of prolyl-4-hydroxylase, an enzyme crucial for collagen synthesis by catalyzing the formation of 4-hydroxyproline residues in collagen molecules. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of P4Hα2 in the sample.

P4Hα2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.120 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Prolyl-4-hydroxylase α polypeptide 2

P4Hα2; Prolyl-4-hydroxylase α polypeptide 2; Alpha polypeptide II

115, 264

The quantitative human P4Hα2 (glycosylated) sandwich ELISA kit utilizes a sandwich ELISA format, allowing researchers to measure P4Hα2 levels, which is valuable in studies related to collagen biosynthesis, fibrosis, and cancer progression. This assay serves as a tool for investigating the role of P4Hα2 in various physiological and pathological processes.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.