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Quantitative Human PLOD1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX522)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PLOD1 (glycosylated) sandwich ELISA kit is designed to detect human procollagen lysine-1,2-oxoglutarate-5-dioxygenase 1 (PLOD1) levels. PLOD1 is an enzyme that modifies collagen, which catalyzes the hydroxylation of lysine residues in procollagen molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.098 ng/mL, which can accurately detect low concentrations of PLOD1 in the sample.
Target PLOD1
N-Glycosylation Site 163, 197, 538, 686
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.098 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Procollagen lysine-1,2-oxoglutarate-5-dioxygenase 1
Alternate Names PLOD1; Procollagen lysine-1,2-oxoglutarate-5-dioxygenase 1; PLOD; LH1; Lysyl hydroxylase 1
Uniprot No. Q02809
Application The quantitative human PLOD1 (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and connective tissue biology, particularly in studies of collagen synthesis and metabolism. Researchers use it to measure PLOD1 levels in tissue samples. The kit has applications in studies of fibrosis, skeletal disorders, and other conditions involving collagen abnormalities.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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