Quantitative Human PLOD1 (Glycosylated) Sandwich ELISA Kit

The quantitative human PLOD1 (glycosylated) sandwich ELISA kit is designed to detect human procollagen lysine-1,2-oxoglutarate-5-dioxygenase 1 (PLOD1) levels. PLOD1 is an enzyme that modifies collagen, which catalyzes the hydroxylation of lysine residues in procollagen molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.098 ng/mL, which can accurately detect low concentrations of PLOD1 in the sample.

PLOD1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.098 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Procollagen lysine-1,2-oxoglutarate-5-dioxygenase 1

PLOD1; Procollagen lysine-1,2-oxoglutarate-5-dioxygenase 1; PLOD; LH1; Lysyl hydroxylase 1

163, 197, 538, 686

The quantitative human PLOD1 (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and connective tissue biology, particularly in studies of collagen synthesis and metabolism. Researchers use it to measure PLOD1 levels in tissue samples. The kit has applications in studies of fibrosis, skeletal disorders, and other conditions involving collagen abnormalities.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.