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Quantitative Human PSCA (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX60

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Specifications
Product Description
The quantitative human PSCA (glycosylated) sandwich ELISA kit is designed to detect human prostate stem cell antigen (PSCA) levels. PSCA is a glycosylphosphatidylinositol-anchored cell surface protein highly expressed in prostate tissue and overexpressed in several cancers, including prostate, bladder, and pancreatic cancers. It is involved in cell proliferation, adhesion, and survival, making it a potential biomarker and therapeutic target in oncology. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.091 ng/mL, which can accurately detect low concentrations of PSCA in the sample.
Target
PSCA
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.091 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Prostate stem cell antigen
Alternate Names
PSCA; Prostate stem cell antigen
Uniprot No.
N-Glycosylation Site
31
Application Notes
Application
The quantitative human PSCA (glycosylated) sandwich ELISA kit is used for the precise measurement of glycosylated PSCA levels in human biological samples. This highly sensitive and specific assay enables researchers to assess PSCA expression quantitatively, facilitating studies on tumor progression, metastasis, and patient response to therapies.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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