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Quantitative Human TRPM8 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX663

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Specifications
Product Description
The quantitative human TRPM8 (glycosylated) sandwich ELISA kit is designed to detect human transient receptor potential cation channel subfamily M, member 8 (TRPM8) levels. TRPM8 is an ion channel protein that is activated by cold temperatures and cooling agents like menthol. This channel is expressed in sensory neurons and plays a crucial role in cold sensation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.133 ng/mL, which can accurately detect low concentrations of TRPM8 in the sample.
Target
TRPM8
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.133 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Transient receptor potential cation channel subfamily M, member 8
Alternate Names
TRPM8; Transient receptor potential cation channel subfamily M, member 8; LTRPC6; TRPP8; CMR1; Long transient receptor potential channel 6; Transient receptor potential p8
Uniprot No.
N-Glycosylation Site
934
Application Notes
Application
The quantitative human TRPM8 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated TRPM8 levels in biological samples. The kit is applicable in studies of sensory biology, cold sensation, and pain. It is useful in fields such as neurobiology and pharmacology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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