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Quantitative Rat VEGFA (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1193

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative rat VEGFA (glycosylated) sandwich ELISA kit is designed to detect rat vascular endothelial cell growth factor A (VEGFA) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 1.051 pg/mL, which can accurately detect low concentrations of VEGFA in the sample.
Target
VEGFA
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated VEGF antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
1.051 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
4 pg/mL-250 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Rat
Full Name
Vascular endothelial growth factor A
Alternate Names
Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF
Uniprot No.
P16612
N-Glycosylation Site
100
Application Notes
Application
The quantitative rat VEGFA (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of VEGFA, helping to understand its role in cancer.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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