Recombinant Human F10 Protein, Glycoengineered Pichia pastoris

Recombinant Human F10 protein (NP_000495.1) is produced in Glycoengineered Pichia pastoris. This expression system is used for the production of glycoproteins and antibodies with human post-translational modifications.

Glycoengineered Pichia pastoris

Human

His-tag, FC-tag

>90%, determined by SDS-PAGE.

Regular batch

None

F10

Coagulation factor X

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

F10; coagulation factor X; FX; FXA