GlycoErase™ PDK3 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF658)

Description	GlycoErase™ PDK3 knockout HepG2 cell line eliminates PDK3, a PDH kinase isoform most active in muscle and heart tissues, where it inhibits the PDH complex. This knockout supports research on tissue-specific glucose oxidation, metabolic flexibility, and cross-talk between hepatic and muscle energy pathways.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PDK3
Full Name	Pyruvate Dehydrogenase Kinase 3
Alternative Name	CMTX6; GS1-358P8.4
Location	Xp22.11
Gene ID	5165
Summary	The protein encoded here is one of three pyruvate dehydrogenase kinases that inhibit the PDH complex by phosphorylating the E1 alpha subunit, thereby controlling the link between glycolysis and the TCA cycle. This gene is mainly expressed in heart and skeletal muscle. Several isoforms exist due to alternative splicing.