GlycoErase™ PHKA1 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF667)

Description	GlycoErase™ PHKA1 knockout HepG2 cell line eliminates PHKA1, encoding the skeletal muscle isoform of the alpha subunit of phosphorylase kinase, a complex regulating glycogen breakdown. Mutations in PHKA1 cause glycogen storage disease type IXD. This knockout provides a liver model to study glycogenolysis, calcium regulation, and inherited glycogen disorders.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKA1
Full Name	Phosphorylase Kinase Regulatory Subunit Alpha 1
Alternative Name	PHKA
Location	Xq13.1
Gene ID	5255
Summary	Phosphorylase kinase is composed of alpha, beta, gamma, and delta subunits. The alpha subunit in skeletal muscle is encoded by this gene. Mutations result in glycogen storage disease type 9D (X-linked muscle glycogenosis). Alternative splicing produces different isoforms, and a pseudogene exists on chromosome 1.