GlycoErase™ SIGLEC1 Knockout HepG2 Cell Line

GlycoErase™ SIGLEC1 knockout HepG2 cell line is a human hepatocellular carcinoma (HepG2) cell line in which SIGLEC1 expression has been eliminated. SIGLEC1 is a sialic acid-dependent adhesion receptor involved in immune regulation and pathogen entry, including viral enhancement. The knockout of SIGLEC1 in the HepG2 background provides a liver-relevant model for studying glycan-mediated interactions, viral infection mechanisms, and immune modulation, and supports applications in drug metabolism research, hepatocellular disease studies, and biomarker discovery.

KO Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Glutamine

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC1

Sialic Acid Binding Ig Like Lectin 1

CD169, Sialic acid-binding Ig-like lectin 1, SIGLEC1, sialoadhesin, Sialic Acid Binding Ig Like Lectin

20p13

This gene encodes a protein from the immunoglobulin superfamily, acting as a lectin-like adhesion molecule. The protein binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed by a subpopulation of macrophages and is involved in mediating cell-cell interactions. The protein plays a crucial role in several human diseases, bacterial and viral infections, and has been shown to enhance SARS-CoV-2 infection.