GlycoErase™ SIGLEC1 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF842)

Description	GlycoErase™ SIGLEC1 knockout HepG2 cell line is a human hepatocellular carcinoma (HepG2) cell line in which SIGLEC1 expression has been eliminated. SIGLEC1 is a sialic acid-dependent adhesion receptor involved in immune regulation and pathogen entry, including viral enhancement. The knockout of SIGLEC1 in the HepG2 background provides a liver-relevant model for studying glycan-mediated interactions, viral infection mechanisms, and immune modulation, and supports applications in drug metabolism research, hepatocellular disease studies, and biomarker discovery.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC1
Full Name	Sialic Acid Binding Ig Like Lectin 1
Alternative Name	CD169, Sialic acid-binding Ig-like lectin 1, SIGLEC1, sialoadhesin, Sialic Acid Binding Ig Like Lectin
Location	20p13
Gene ID	6614
Summary	This gene encodes a protein from the immunoglobulin superfamily, acting as a lectin-like adhesion molecule. The protein binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed by a subpopulation of macrophages and is involved in mediating cell-cell interactions. The protein plays a crucial role in several human diseases, bacterial and viral infections, and has been shown to enhance SARS-CoV-2 infection.