GlycoErase™ SIGLEC1 Knockout Jurkat Cell Line (CAT#: GLJF-0825-JF843)

Description	GlycoErase™ SIGLEC1 knockout Jurkat cell line is a human T lymphocyte leukemia (Jurkat) cell line in which SIGLEC1 expression has been eliminated. SIGLEC1 is a sialic acid-dependent adhesion receptor involved in immune regulation and pathogen entry. The knockout of SIGLEC1 in the Jurkat background provides an immune-relevant model for studying glycan-mediated interactions, T cell signaling pathways, and immune modulation, and supports applications in immunotherapy research, cytokine regulation studies, and host-pathogen investigations.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC1
Full Name	Sialic Acid Binding Ig Like Lectin 1
Alternative Name	CD169, Sialic acid-binding Ig-like lectin 1, SIGLEC1, sialoadhesin, Sialic Acid Binding Ig Like Lectin
Location	20p13
Gene ID	6614
Summary	This gene encodes a protein from the immunoglobulin superfamily, which functions as a lectin-like adhesion molecule. It binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. This type I transmembrane protein is expressed by a subset of macrophages and plays a critical role in mediating cell-cell interactions. The protein is involved in various human diseases and infections, and it has been shown to enhance SARS-CoV-2 infection.