GlycoErase™ SIGLEC6 Knockout A549 Cell Line (CAT#: GLJF-0825-JF892)

Description	GlycoErase™ SIGLEC6 knockout A549 cell line is a human lung epithelial cancer model with SIGLEC6 expression removed. SIGLEC6 is a transmembrane lectin receptor that recognizes sialyl-TN glycans and also binds leptin. Its expression is notably upregulated in the placenta during preeclampsia, highlighting its relevance in glycan recognition and pregnancy-related disorders. The knockout of SIGLEC6 in the A549 background provides an effective system to study glycan-protein interactions, metabolic signaling involving leptin, and disease processes such as preeclampsia, while supporting drug discovery, biomarker exploration, and translational research in reproductive and metabolic biology.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:3~1:6
Cell Line	A549
Primary Disease	Non-small cell lung cancer
Lineage	Lung
Lineage Subtype	Non-small cell lung cancer

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	F-12K & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC6
Full Name	Sialic Acid Binding Ig Like Lectin 6
Alternative Name	CD327, CD33L, OBBP1, CD33L1, CD33L2, CDW327, Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	946
Summary	This gene encodes a member of the SIGLEC (sialic acid binding immunoglobulin-like lectin) family of proteins. The encoded transmembrane receptor binds sialyl-TN glycans and leptin. Placental expression of the encoded protein is upregulated in preeclampsia.