GlycoErase™ GBE1 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF398)

Description	GlycoErase™ GBE1 knockout HEK293 cell line is a human embryonic kidney (HEK293) model in which GBE1 expression has been eliminated. GBE1 encodes glycogen branching enzyme that generates alpha-1,6 branches in glycogen, critical for solubility and energy balance. Mutations in GBE1 cause glycogen storage disease IV (Andersen's disease). This knockout HEK293 model provides a platform to study glycogen branching, storage disorders, and energy regulation in human cells, while supporting therapeutic target validation for glycogen metabolism diseases.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GBE1
Full Name	1,4-Alpha-Glucan Branching Enzyme 1
Alternative Name	GBE; APBD; GSD4
Location	3p12.2
Gene ID	2632
Summary	The protein encoded by this gene is a glycogen branching enzyme that transfers alpha-1,4-linked glucosyl units to create branches in glycogen chains, increasing solubility and reducing osmotic pressure. This enzyme is primarily expressed in the liver and muscle, and mutations lead to glycogen storage disease IV (Andersen's disease).