GlycoSens™ Acetolactate Synthase (ALS) Assay Kit

The GlycoSens™ Acetolactate Synthase (ALS) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target acetolactate synthase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Nitrogen Metabolism Series

ALS

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of acetolactate synthase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect ALS content in the sample.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Acetolactate Synthase

Acetolactate Synthase; ALS; Acetolactate Synthetase

Research highlights acetolactate synthase (ALS) as a significant participant in key metabolic and regulatory cascades. It orchestrates the biosynthesis of complex biological molecules from simpler precursors. Notably, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Designed for comprehensive functional analysis of acetolactate synthase (ALS) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Linear Dynamic Range; Excellent Reproducibility; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.