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GlycoSens™ Acetolactate Synthase (ALS) Assay Kit, Spectrophotometry

CAT#: GLJF-0926-HX1458

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Specifications
Product Description
The GlycoSens™ Acetolactate Synthase (ALS) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of acetolactate synthase (ALS) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Other Series
Target
ALS
Kit Components
Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
5 μL - 20 μL
Detection Principle
Quantification of acetolactate synthase (ALS) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Acetolactate Synthase
Alternate Names
Acetolactate Synthase; ALS; Acetolactate Synthetase
Application Notes
Background
In the context of biological research, acetolactate synthase (ALS) has emerged as a molecule of high interest. It orchestrates the biosynthesis of complex biological molecules from simpler precursors. Moreover, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.
Application
Ideal for investigating acetolactate synthase (ALS) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
Key Features & Advantages
Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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