GlycoSens™ Formate Dehydrogenase (FDH) Assay Kit, Spectrophotometry

The GlycoSens™ Formate Dehydrogenase (FDH) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of formate dehydrogenase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Other Series

FDH

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

Target formate dehydrogenase (FDH) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target FDH.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Formate Dehydrogenase

Acid Dehydrogenase; FDH; Acid Oxidoreductase; Formate Dehydrogenase

As a pivotal biomolecule, formate dehydrogenase (FDH) plays a central role in complex physiological networks. It is responsible for driving the oxidative conversion of its substrate, typically utilizing NAD or FAD as cofactors. Moreover, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Tailored for accurate quantification of formate dehydrogenase (FDH) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.