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GlycoSens™ Formate Dehydrogenase (FDH) Assay Kit

CAT#: GLJF-0926-HX420

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The GlycoSens™ Formate Dehydrogenase (FDH) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of formate dehydrogenase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Nitrogen Metabolism Series
Target
FDH
Kit Components
Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
Target formate dehydrogenase (FDH) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target FDH.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Formate Dehydrogenase
Alternate Names
Acid Dehydrogenase; FDH; Formate Dehydrogenase
Application Notes
Background
Known for its specific biological characteristics, formate dehydrogenase (FDH) provides deep insights into cellular dynamics. This enzyme facilitates essential dehydrogenation steps required for cellular respiration and energy production. Assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Specifically engineered for evaluating formate dehydrogenase (FDH) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
Key Features & Advantages
High Specificity; Micro-Volume Optimization; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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