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The GlycoSens™ Formaldehyde Dehydrogenase (FDH) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of formaldehyde dehydrogenase (FDH) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Coenzyme I Series
Target
FDH
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
Quantification of formaldehyde dehydrogenase (FDH) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
As a pivotal biomolecule, formaldehyde dehydrogenase (FDH) plays a central role in complex physiological networks. By mediating the transfer of electrons, it strictly regulates intermediate metabolic fluxes. Notably, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.
Application
Ideal for investigating formaldehyde dehydrogenase (FDH) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.