GlycoSens™ Mitochondrial Respiratory Chain Complex I Assay Kit, Spectrophotometry

The GlycoSens™ Mitochondrial Respiratory Chain Complex I Assay Kit offers a highly reliable and sensitive spectrophotometric platform for quantifying mitochondrial respiratory chain complex I across varied biological matrices. Designed for rapid operation and high precision, this assay ensures reproducible quantification with low sample volume requirements.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex I

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

10 μL - 50 μL

Complex I catalyzes NADH dehydrogenation to yield NAD⁺. The oxidation rate of NADH is continuously monitored by tracking optical density loss at 340 nm using spectrophotometer.

Within 1 hour

340 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex I

Complex I; Mitochondrial Respiratory Chain Complex I Standard; Mitochondrial Respiratory Chain Complex I Reagent; NADH-Coenzyme Q Reductase

Found widely across biological samples, mitochondrial respiratory chain complex I is crucial for maintaining systemic homeostasis. It plays a critical role in cellular bioenergetics by maintaining the proton motive force across the inner mitochondrial membrane. Consequently, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Designed for comprehensive functional analysis of mitochondrial respiratory chain complex I within molecular biochemistry, cellular energy metabolism, and metabolic pathway flux analysis.

Optimized Reaction Dynamics; Rapid Reaction Protocol; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.