GlycoSens™ Mitochondrial Respiratory Chain Complex I Assay Kit, Micro-method

The GlycoSens™ Mitochondrial Respiratory Chain Complex I Assay Kit provides an advanced, quantitative micro-volume spectrophotometric method for determining mitochondrial respiratory chain complex I in biological specimens. Featuring superior kinetic linearity and high throughput compatibility, it facilitates accurate biochemical profiling.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex I

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

10 μL - 50 μL

Complex I catalyzes NADH dehydrogenation to yield NAD⁺. The oxidation rate of NADH is continuously monitored by tracking optical density loss at 340 nm using microplate reader.

Within 1 hour

340 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex I

Complex I; Mitochondrial Respiratory Chain Complex I Standard; Mitochondrial Respiratory Chain Complex I Reagent; NADH-Coenzyme Q Reductase

Mitochondrial respiratory chain complex I acts as an essential biochemical entity within diverse cellular pathways. It functions as an essential multi-subunit structure within the mitochondrial electron transport chain. Thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Designed for comprehensive functional analysis of mitochondrial respiratory chain complex I within molecular biochemistry, NADH oxidation rate, and metabolic pathway flux analysis.

Stable Signal Output; Broad Sample Compatibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.