GlycoSens™ Pectinase Assay Kit

The GlycoSens™ Pectinase Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of pectinase levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

Pectinase

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Isolated mitochondria, cell lysates, tissue homogenates

10 μL - 50 μL

Quantification of pectinase is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

540 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pectinase

Pectinase

As a pivotal biomolecule, pectinase plays a central role in complex physiological networks. This catalytic protein strictly regulates substrate turnover to meet the dynamic physiological needs of the cell. Assessing this component offers a reliable parameter for agricultural research or biological studies.

Designed for comprehensive functional analysis of pectinase within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.