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The GlycoSens™ Pectinase Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of pectinase levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Pectin Series
Target
Pectinase
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
5 μL - 20 μL
Detection Principle
Quantification of pectinase is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Pectinase
Alternate Names
Pectinase
Application Notes
Background
Research highlights pectinase as a significant participant in key metabolic and regulatory cascades. This catalytic protein strictly regulates substrate turnover to meet the dynamic physiological needs of the cell. As a result, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.
Application
Widely applicable for tracking pectinase fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.